Performing lineagespecific chimerism analysis can increase the sensitivity of assays and provide essential information. Both of these benefits depend upon the purity of isolated cell subsets, as contamination by nontarget cells decreases the reliability of lineagespecific analysis. Molecular analysis of lineagespecific chimerism and minimal residual disease by RTPCR of p210 BCRABL and p190 BCRABL after allogeneic bone marrow transplantation for chronic myeloid leukemia: increasing mixed myeloid chimerism and p190 BCRABL detection precede cytogenetic relapse Mar 15, 2011 Keywords: Lineagespecific chimerism, Myeloablative stem cell transplantation, T cells, Natural killer cells INTRODUCTION Chimerism analysis is an important tool used to assess the origin of hematopoietic cells after allogeneic stem cell transplantation (alloSCT).
Request PDF on ResearchGate Detection of Impending Graft Rejection and Relapse by LineageSpecific Chimerism Analysis Molecular surveillance of hematopoietic chimerism has become part of the Chimerism analysis in lineagespecific cells permits detection of relapse and facilitates the monitoring of therapeutic interventions.
These results can provide the basic data for chimerism analysis after myeloablative SCT. Key Words Lineagespecific chimerism, Myeloablative stem cell transplantation, T This study has confirmed that lineagespecific chimerism analysis represents a valuable adjunct to our whole blood studies.
Due to its increased sensitivity it can be used to reveal mixed chimerism in specific leukocyte populations that are masked in the whole blood analysis. Information about the relative Chimerism analysis within 6 months of allogeneic stem cell transplantation predicts relapse in acute myeloid leukemia lineagespecific chimerism analysis is a sensitive predictor of relapse in Failure to achieve complete donor Tcell chimerism in the early posttransplant period may impair the graftversus malignancy effect and contribute to a higher risk of disease relapse.
Lineage specific chimerism analysis (LSCA) of Tlymphocytes versus myeloid cells is routinely performed following reducedintensity or nonmyeloablative